| Clonality | monoclonal |
| Host | Mouse |
| Specificity | Human, Mouse |
| Tested Application | ELISA, WB, IHC, IF |
| Delivery Time | 2 to 4 working days |
| Clone ID | 8D5 |
| Isotype | IgG1 |
| Form | liquid |
| Purification | Protein A+G purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q15942 |
| Calculated MW | 80 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin(ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. |
| Immunogen | zyxin |
| Synonyms | ESP 2, HED 2, ZYX, zyxin, Zyxin 2 |
| Recommended dilution | WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200 |
Immunohistochemistry of paraffin-embedded human kidney slide using FNab09765(ZYX Antibody) at dilution of 1:50
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HeLa cells were subjected to SDS PAGE followed by western blot with FNab09765(ZYX antibody) at dilution of 1:1000
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