Bovine Serum Albumin (BSA)

Bovine Serum Albumin (BSA) is a high purity, lyophilized “Fraction V” powder derived from the plasma of USDA-inspected healthy animals located in the USA. Bovine Serum albumin is isolated from other plasma protein products and lipids by a unique proprietary, non-solvent procedure. The purification process includes a modified Cohn/heat-shock procedure designed to inactivate proteases and other potentially interfering enzymes. Further processing such as extensive membrane dialysis and filtration, minimizes analytes that can cause background interference in sensitive assays as well as inhibition in sensitive cell and microbial culture systems.

Bovine Serum Albumin is applicable for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Frequently used as a blocking reagent; particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.

APPLICATIONS:
⋅ Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent
⋅ Protein Standard, Diluent
⋅ Protein, Conjugate or Enzyme Stabilizer
⋅ Hybridization
⋅ Selected Cell Culture applications

FEATURES AND BENEFITS:
⋅ High Purity, Low Interference, Low Background
⋅ Virtually No Detectable IgG, Endotoxin, Enzyme or Protease Activity
⋅ Superior Solubility/Filterability
⋅ Validated for Prion (BSE/TSE) Clearance
⋅ Endotoxin (LAL): ≤ 0.1EU/mg max

Appearance:	Lyophilized powder
Physical State:	Solid
Solubility:	Soluble in water (40 mg/ml).
Storage:	Desiccate at 4° C

WGK Germany:	3
RTECS:	MT6446000
Merck Index:	14: 8468
MDL Number:	MFCD00130384
EC Number:	232-936-2