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Human Keratin, type I cytoskeletal 14, KRT14 ELISA Kitkeratin 14 (KRT14), ELISA Kit
| Product Name |
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keratin 14 (KRT14), ELISA Kit
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| Also Known As |
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Human Keratin, type I cytoskeletal 14, KRT14 ELISA Kit
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| Product Synonym Names |
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Human Keratin; type I cytoskeletal 14 (KRT14) ELISA kit; CK14; EBS3; EBS4; K14; NFJ; cytokeratin 14; keratin 14 (epidermolysis bullosa simplex; Dowling-Meara; Koebner) ; keratin 14 |
| Product Gene Name |
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KRT14 elisa kit
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| Species Reactivity |
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Human |
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| Specificity |
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This assay has high sensitivity and excellent specificity for detection of Human KRT14. No significant cross-reactivity or interference between Human KRT14 and analogues was observed. |
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| Intra-assay Precision |
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Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess. |
| Detection Wavelength |
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450 nm |
| Sample Volume |
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50-100ul |
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| Preparation and Storage |
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. |
| Other Notes for KRT14 elisa kit |
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Small volumes of KRT14 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice. |
Product Description specifically for KRT14 elisa kit
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Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KRT14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for KRT14 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain KRT14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of KRT14 in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Информация для заказа| Область использования: | Производство: | --- | | Метод: | | | Объем: | | | Кат. номер: | MBS914821 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Human Keratin, type I cytoskeletal 14, KRT14 ELISA Kit / keratin 14 (KRT14), ELISA Kit.
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