Моноклональные антитела к Альфа- фетопротеину человека, PE

Human/Mouse alpha-Fetoprotein Phycoerythrin MAb

alpha-Fetoprotein (AFP), a member of the albuminoid superfamily, is a fetal/tumor-associated protein that is a marker for certain cancers and congenital defects. Like other members of the albuminoid family, AFP can act as a carrier protein, binding several types of molecules including steroids, bilirubin, fatty acids, retinoids, and flavanoids. Some studies suggest that AFP may also act as a regulator of cell growth and survival.

DESCRIPTION

Species Reactivity Human/Mouse

Specificity	Detects human a-Fetoprotein/AFP in direct ELISAs and Western blots.
Source	Monoclonal Mouse IgG₁ Clone # 189502
Purification	Protein A or G purified from hybridoma culture supernatant
Immunogen	Human umbilical cord serum-derived a-Fetoprotein/AFP

Formulation Lyophilized from a 0.2 |jm filtered solution in PBS with Trehalose. See

APPLICATIONS

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

	Recommended Concentration	Sample
Western Blot	1 jg/mL	Human a-Fetoprotein/AFP
Immunocytochemistry	8-25 jg/mL	Immersion fixed HepG2 human hepatocellular carcinoma cell line and NCTC 1469 mouse liver cell line

Intracellular Staining by Flow Cytometry 2.5 |jg/10⁶ cells HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and

permeabilized with saponin

PREPARATION AND STORAGE

Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month from date of receipt, 2 to 8 °C, reconstituted.
6 months from date of receipt, -20 to -70 °C, reconstituted.

BACKGROUND

a-Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors.

Информация для заказа

Область использования: Моноклональные антитела и конъюгаты для проточной цитометрии	Производство:	R&D
	Метод:	Проточная цитометрия
	Объем:	100 тестов
	Кат. номер:	IC1368P
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Моноклональные антитела к Альфа- фетопротеину человека, PE / Human/Mouse alpha-Fetoprotein Phycoerythrin MAb . Примечание: