Антитела Anti-C3d [7C10], мышиные моноклональные

• Product nameAnti-C3d antibody [7C10]
  See all C3d primary antibodies
• Description
  Mouse monoclonal [7C10] to C3d
• Tested applicationsIHC, ELISA, WB, Flow Cytmore details
• Species reactivity
  Reacts with: Human
• Immunogen

  Full length native protein (purified) corresponding to Human C3d.

• EpitopeEpitope specificity differs from that of ab17454 and ab17455.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
• Storage bufferpH: 7.40
  Preservative: 0.098% Sodium azide
  Constituents: PBS, 2.9% Sodium chloride
• Concentration100 µg at 1 mg/ml 
  
• PurityProtein A purified
• ClonalityMonoclonal
• Clone number7C10
• Myelomax63-Ag8.653
• IsotypeIgG1
• Light chain typekappa

Applications

Target

• FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
  Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
• Tissue specificityPlasma.
• Involvement in diseaseDefects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
  Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
• Sequence similaritiesContains 1 anaphylatoxin-like domain.
  Contains 1 NTR domain.
• Post-translational
  modificationsC3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
  Phosphorylation sites are present in the extracelllular medium.
• Cellular localizationSecreted.
• Information by UniProt
• Database links
  • Entrez Gene: 718 Human
  • Omim: 120700 Human
  • SwissProt: P01024 Human
  • Unigene: 529053 Human
• Alternative names
  • Acylation stimulating protein cleavage product antibody
  • AHUS5 antibody
  • ARMD9 antibody

  Anti-C3d antibody [7C10] images

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    Immunohistochemistry - Anti-C3d antibody [7C10] (ab17453)

    Immunohistochemical analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.

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    Immunofluorescence - Anti-C3d antibody [7C10] (ab17453)

    Immunofluorescence analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.

  References for Anti-C3d antibody [7C10] (ab17453)

  This product has been referenced in:

  • Le Friec G  et al. The CD46-Jagged1 interaction is critical for human T(H)1 immunity. Nat Immunol 13:1213-21 (2012). Read more (PubMed: 23086448) »