Anti-Vimentin antibody [RV202] - Cytoskeleton Marker
Mouse monoclonal [RV202] to Vimentin Specificity This antibody reacts exclusively with vimentin Tested applications Flow Cyt, ICC, IHC-Fr, WB, IHC-FoFr, IP, IHC-P, ICC/IF, IHC (PFA fixed)
Species reactivity Reacts with: Mouse, Rat, Goat, Chicken, Hamster, Cow, Dog, Human, Monkey, Zebrafish
Immunogen Fusion protein corresponding to Human Vimentin. RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a vimentin extract of bovine lens. Positive control Purchase matching WB positive control: Human Vimentin full length protein tonsilar lymphoma, cultured bovine lens epithelial cells
Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Avoid freeze / thaw cycle. Storage buffer Preservative: 0.09% Sodium
Azide Constituents: PBS Concentration 100 µg at 1 mg/ml
Purity Protein G purified Clonality Monoclonal Clone number RV202
Myeloma Sp2/0-Ag14 Isotype IgG1 Research Areas Neuroscience Cell Type Marker Neural Stem Cell marker Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Vimentin Neuroscience Neurology process Neurogenesis Stem Cells Lineage Markers Endoderm Stem Cells Neural Stem Cells Intracellular Cancer Tumor biomarkers Other Neuroscience Neurology process Neuroregeneration Neuroregeneration Developmental Biology Lineage Specification Ectoderm Applications Our Abpromise guarantee covers the use of ab8978 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes Flow Cyt 1/100 - 1/200. ICC 1/20. IHC-Fr Use at an assay dependent concentration. Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection. For PFA fixed tissue use at 1/1000. WB 1/100 - 1/1000. Detects a band of approximately 57 kDa. IHC-FoFr 1/500. IP Use at an assay dependent concentration. IHC-P Use at
an assay dependent concentration. ICC/IF Use at an assay dependent concentration. PubMed: 20097175 IHC (PFA fixed) 1/1000.
Информация для заказа
Область использования: | Производство: | Abcam |
Метод: | |
Объем: | 100 мкг |
Кат. номер: | ab8978 |
Цена (с НДС 20%): | по запросу | В корзину |
Наименование: Anti-Vimentin antibody [RV202] - Cytoskeleton Marker. Примечание: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Дополнительная информация (на английском). |