Hoechst 33342 trihydrochloride (CAS 23491-52-3)

Synonym:BisbenzimideApplication:An A:T-specific DNA minor groove ligand used for visualizing cellular DNACAS Number:23491-52-3Purity:95%Molecular Weight:561.9Molecular Formula:C₂₇H₂₈N₆O 3HClRefer to Certificate of Analysis for lot specific data (including water content).

Description

Hoechst 33342 trihydrochloride is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin (sc-200923) has been shown to disrupt the DNA dye update.

The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342’s fluorescent capability, the chemical has also had varying effects on differing cell types. The stain has been shown to induce cell differentiation, bind reversibly to Friend erythroleukemia cells, inhibit Topo I (topoisomerase I), induce apoptosis in HL-60 cells, and in junctional sarcoplasmic reticulum cells regulate Ca²⁺ efflux and affect cell differentiation.

Technical Information

Physical State:	Solid
Solubility:	Soluble in water (20 mg/ml). Precipitates in phosphate buffered solutions
Storage:	Store at -20° C
Fluoresence:	λex 346 nm, λem 460 nm

Safety and Reference Information

RTECS:	DT4200000
PubChem CID:	16760503
Merck Index:	14: 10367
MDL Number:	MFCD00012678
SMILES:	CCOC1=CC=C(C=C1)C2=NCC3=C(N2)C=CC(=C3)C4=NCC5=C(N4)C=CC(=C5)N6CCN(CC6)C.Cl.C l.Cl

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

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9. Crissman, H.A., et al. 1990. Methods Cell Biol. 33: 97-103. PMID: 1707496
10. Ormerod, M.G., et al. 1993. Cytometry. 14: 595-602. PMID: 8404365
11. Wong, S.S., et al. 1994. Biochem. Pharmacol. 47: 827-837. PMID: 7510966
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13. Zhang, X. and Kiechle, F. 2001. Ann. Clin. Lab. Sci. 31: 187-198. PMID: 11337909

Информация для заказа

Область использования: Реагенты SantaCruz	Производство:	Santa Cruz
	Метод:
	Объем:	100 мг
	Кат. номер:	SC-200 908
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Hoechst 33342 trihydrochloride (CAS 23491-52-3). Примечание: