Набор для анализа холестерина / эфира холестерина (Cholesterol/ Cholesteryl Ester Assay Kit Quantitation)

Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation

• Assay type: Quantitative
• Detection method: Colorimetric/Fluorometric
• Platform: Microplate reader
• Assay time: 1 hr
• Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine

• Assay time

  1h 00m

• Product overview

  Cholesterol/ Cholesteryl Ester Assay Kit ab65359 provides a simple method for sensitive quantification of total cholesterol, free cholesterol, and cholesteryl esters, by colorimetric or fluorometric methods.

  
  

  The majority of cholesterol in blood is in the form of cholesteryl esters. In the cholesterol assay protocol, these are hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H₂O₂ which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).

  
  

  The assay detects total cholesterol (cholesterol and cholesteryl esters) when cholesterol esterase is included in the reaction, or free cholesterol when it is not included. The amount of cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).

  
  

  Cholesterol assay protocol summary:
  - add samples and standards to wells
  - add total cholesterol reaction mix (with esterase) or free cholesterol reaction mix (without esterase) and incubate for 60 min at 37ºC
  - analyze with microplate reader

• Notes

  Other cholesterol assay kits include:
  - HDL and LDL/VLDL Cholesterol assay kit ab65390 
  - Cell-based Cholesterol assay kit ab133116 
  - Cholesterol Efflux assay kit ab196985 
  - Cholesterol Uptake assay kit ab236212

  Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

   

  Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
  It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

• Platform

Информация для заказа

Область использования: Реагенты Abcam	Производство:	Abcam
	Метод:
	Объем:	100 test
	Кат. номер:	ab65359
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для анализа холестерина / эфира холестерина (Cholesterol/ Cholesteryl Ester Assay Kit Quantitation) / Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation. Примечание: +4°C Дополнительная информация (на английском).