Набор LEGENDplex(TM) Mouse Inflammation Panel (13-plex) with V-bottom Plate 100 tests LEGENDplex™ Mouse Inflammation Panel (13-plex) with V-bottom PlateDescription
Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells. They regulate immunity, inflammation and hematopoiesis as well as cell growth, migration, development and differentiation. Cytokines act in large tightly regulated networks where both the production and action of one cytokine is affected by the behavior of other cytokines. The accurate measurement of the expression profile of cytokines in a sample is critical to complete and in-depth understanding of disease progression and immune responses.
The LEGENDplex™ Mouse Inflammation Panel is a multiplex assay by using fluorescence–encoded beads suitable for the analysis on various flow cytometers. This panel allows simultaneous quantification of 13 mouse cytokines, including IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17A, IL-23, IL-27, MCP-1, IFN-β, IFN-γ, TNF-α, and GM-CSF. Most of cytokines in this panel are produced by innate immune cells, linking the innate and adaptive immunity and/or bystander cells. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.
- Kit Contents
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- Setup Beads 1: FITC Beads (1 mL)
- Setup Beads 2: PE Beads (1 mL)
- Setup Beads 3: Raw Beads (2 mL)
- Capture Beads
- Detection Antibody (3.5 mL)
- Standard Cocktail, Lyophilized (1 vial)
- SA-PE (3.5 mL)
- Matrix C, Lyophilized (1 vial)
- Assay Buffer (25 mL)
- Wash Buffer (25 mL)
- V-bottom Plate (1 plate)
- Plate Sealers (4 sheets)
- Reactivity
- Mouse
- Application
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Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Product Citations
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- Schierer S, et al. 2018. Life Sci Alliance. 1:e201800093. PubMed
- Farris BY, et al. 2019. Immun Inflamm Dis. 0.518055556. PubMed
- Singh Y, et al. 2019. Front Neurosci. 1.258333333. PubMed
- Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
- Chandran S, et al. 2020. Front Immunol. 11:787. PubMed
- Materials Not Included
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- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Plate Shaker
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
Информация для заказаОбласть использования: | Производство: | Biolegend | Метод: | | Объем: | 100 тестов | Кат. номер: | BLD-740446 | Цена (с НДС 20%): | по запросу | В корзину | Наименование: Набор LEGENDplex(TM) Mouse Inflammation Panel (13-plex) with V-bottom Plate 100 tests / LEGENDplex™ Mouse Inflammation Panel (13-plex) with V-bottom Plate. Примечание: |
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