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/ Каталог / Реагенты для научных исследований / Реагенты Biolegend

Recombinant Human CD19-Fc Chimera

Other Names B-lymphocyte antigen CD19, CD19 Molecule, CD19 Antigen, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, Differentiation Antigen CD19, CVID3
Description

CD19 is a 95 kD transmembrane glycoprotein that functions as a co-receptor for the B-cell antigen receptor (BCR) complex on B lymphocytes. CD19 is expressed throughout B cell development, and it is commonly used as a B-cell lineage marker. CD19 plays an important role in B-cell activation and humoral immune responses. It is required for the responsiveness of mature B cells to antigen stimulation, germinal center development, and antibody affinity maturation. CD19's association with the BCR, CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13 enables CD19 to amplify B-cell signaling and decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. CD19 activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores. CD19 polymorphisms are associated with the development of autoimmunity by promoting autoantibody production. Homozygous mutations in CD19 cause a type of hypogammaglobulinemia in which the response of mature B cells to antigenic stimulation is defective.

 Product Details
 Technical Data Sheet (pdf)

Product Details

Source
Human CD19, amino acid Glu21-Lys291 (Accession # P15391) with a C-terminal IgG1 Fc was expressed in CHO.
Molecular Mass
The 515 amino acid recombinant protein has a predicted molecular mass of approximately 57.5 kD. The DTT-reduced protein migrates at approximately 75 kD and the non-reduced protein migrate at approximately 150 kD by SDS-PAGE. The predicted N-terminal amino acid is Glu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human CD19-Fc Chimera is immobilized at 2 µg/mL, biotinylated human CD81-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 100 - 400 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Covalently linked homodimer
Distribution

Detected on marginal zone and germinal center B cells in lymph nodes, detected on blood B cells

Function
Functions as a co-receptor for the B-cell antigen receptor (BCR) complex on B lymphocytes
Interaction
B cell
Ligand/Receptor
CD21, CD81
Bioactivity
Measured by its ability to bind human CD81
Cell Type
B cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology
Molecular Family
Soluble Receptors
Antigen References
  1. de Rie MA, et al. 1989. Cell Immunol. 118:368-81.
  2. Carter RH, et al. 1992. Science. 256:105-107.
  3. Buhl AM, et al. 1997. J Exp Med. 186:1897-910.
  4. Sato S, et al. 1997. J Immunol. 159:3278-87.
  5. Wang Y, et al. 2002. Immunity. 17:501-14.
  6. van Zelm MC, et al. 2006. N Engl J Med. 354:1901-12.

Информация для заказа

Область использования:Производство:Biolegend
Метод: 
Объем:100 мкг 
Кат. номер:789006
Цена (с НДС 20%):по запросуВ корзину
Наименование: Recombinant Human CD19-Fc Chimera .
Примечание:
   
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info@LD.ru   тел.: +7 495 369-20-43