LEGENDplex™ MU Th Cytokine Panel (12-plex) w/ VbP V03
- Other Names
- Multiplex assay, flow cytometry, inflammation, T helper cell, CBA, cytokines, Th1, Th2, Th9, Th17, Th1/Th2, Th22
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Description
T helper (Th) cells play important roles in regulating immune responses. They secrete cytokines to stimulate various effector cells, such as cytotoxic T cells, B cells and macrophages. These cytokines are also involved in inflammatory and autoimmune diseases. Accurate measurement of Th cytokine expression is critical for in-depth understanding of the immune responses and many disease processes.
The LEGENDplex™ Mouse Th Cytokine Panel is a bead-based multiplex assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 12 Mouse cytokines, including IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 22, IFN-γ and TNF-α, which are collectively secreted by Th1, Th2, Th9, Th17, and Th22. This assay panel provides higher sensitivities and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum and cell culture supernatant samples.
The LEGENDplex™ Mouse Th Panel is designed to allow flexible customization within the panel. It can also be divided into sub-panels, such as:
- LEGENDplex™ MU Th1 Panel (5-Plex)
- LEGENDplex™ MU Th2 Panel (6-Plex)
- LEGENDplex™ MU Th17 Panel (7-Plex)
- LEGENDplex™ MU Th1/Th2 Panel (8-Plex)
- LEGENDplex™ MU Th9 Panel (3-Plex)
- LEGENDplex™ MU Th22 Panel (4-Plex)
Please refer to the target selection table for panel-specific target information. Please visit www.biolegend.com/legendplex for more information on panel design and how to mix and match within the panel.
Product Details
- Kit Contents
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- Setup Beads 1: FITC Beads (1 mL)
- Setup Beads 2: PE Beads (1 mL)
- Setup Beads 3: Raw Beads (2 mL)
- Capture Beads (Varies)
- Mouse Th Panel Detection Antibodies (3.5 mL)
- Mouse Th Panel Standard Cocktail, Lyophilized (1 vial)
- LEGENDplex™ SA-PE (3.5 mL)
- LEGENDplex™ Matrix B, Lyophilized (1 vial)
- LEGENDplex™ Assay Buffer (25 mL)
- LEGENDplex™ Wash Buffer, 20X (25 mL)
- Plate Sealers (4 sheets)
- V-bottom Plate
- Reactivity
- Mouse
- Application
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Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
Materials Not Included
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- Manual
Информация для заказаОбласть использования: | Производство: | Biolegend | Метод: | | Объем: | | Кат. номер: | 741044 | Цена (с НДС 20%): | по запросу | В корзину | Наименование: LEGENDplex™ MU Th Cytokine Panel (12-plex) w/ VbP V03. Примечание: |
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