Набор SARS-CoV-2 Inhibitor Screening Kit

• Background
  The long terminal repeat retrotransposon-derived protein PEG10 binds RNA and also forms capsids of virus-like particle. PEG10 prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. PEG10 inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ACVRL1. PEG10 may bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
• Application
  This kit is useful for screening inhibitors of SARS-CoV-2.
  ELISA Buffer Set-96T (EBS-001) is sold separately and not included in kit, and please contact us if you need the buffer.
• Reconstitution
  See Certificate of Analysis for details of reconstitution instruction and specific concentration.
• Storage

  Upon receipt, please store all items at -20℃ to -70℃. After reconstitution, the stock solution should be kept at -70℃.

  It is recommended not to freeze thaw more than 3 times.

  This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; ●-20℃ to -70℃ for 1 year in lyophilized state; ● -70℃ for 6 months under sterile conditions after reconstitution

• Assay Principles
  This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized SARS-CoV-2 S protein RBD and in-house developed biotinylated Human ACE2 protein. This product is uniquely suitable for rapid high-throughput screening of SARS-CoV-2 inhibitors. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an SARS-CoV-2 inhibitor (as method verified Reference), and Streptavidin-HRP reagent.

  Your experiment will include 4 simple steps:

  a) Coat the plate with SARS-CoV-2 S protein RBD.

  b) Add 50ul biotinylated human ACE2 to coated plate.

  c) Add the reference or your molecule of interest.

  d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

  Finally, the ability of your compound to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.

   

  Materials Provided

  ID	Components	Size	Size
  A010-214	SARS-CoV-2 Spike RBD	10 μg	60 μg
  A011-214	Biotinylated Human ACE2	10 μg	10 μg
  A003-214	Streptavidin-HRP	10 μg	10 μg
  AC2-NA005	SARS-CoV-2 Inhibitor (Human ACE2)	20 μg	50 μg

Информация для заказа

Область использования: ИФА наборы Acro Biosystems	Производство:	Acro Biosystems
	Метод:
	Объем:
	Кат. номер:	EP-105-96tests-AC
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор SARS-CoV-2 Inhibitor Screening Kit. Примечание: