Рекомбинантный белок SARS-CoV-2 (2019-nCoV) Spike S1-mFc Recombinant Protein - 100 ug (Meridian Life Science )

Purity

> 90 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1, mFc Tag)(40591-V05H1), the EC₅₀ of 2019-nCoV Spike Protein (S1, mFc Tag) is 90-240 ng/mL.

Protein Construction

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Accession#

Expressed Host

HEK293 Cells

Species

SARS-CoV-2

Predicted N Terminal

Val16

Molecule Mass

The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, mFc Tag) consists of 904 amino acids and predicts a molecular mass of 101.4 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.