Рекомбин. белок SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, His Tag)

> 95 % as determined by SDS-PAGE

< 1.0 EU per μg of the protein as determined by the LAL method

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag)(Cat: 40592-V08H), the EC₅₀ of SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag)(Cat: 40592-V08H) is 20-60 ng/mL.

Protein Construction

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.

Accession#

Expressed Host

HEK293 Cells

Species

SARS-CoV-2

Predicted N Terminal

Arg319

Molecule Mass

The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 26.54 kDa.

Formulation

Lyophilized from sterile PBS, PH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.