RuV KlS, native virus

Native

Unconjugated

Vera cells infected with Rubella release virus particles into the culture supernatant. Supernatant is harvested and clarified. The material is then concentrated by crossflow ultrafiltration. The virus suspension is then purified by sucrose density gradien

None

1 mL;10 mL

None

Store this antigen preparation frozen at -70 °C to -100 °C. Repeated freezing and thawing should be avoided.

Rubella virus is classified as a togavirus, genus Rubivirus. It is most closely related to group A arboviruses, such as Eastern and Western Equine Encephalitis viruses. It is an enveloped RNA virus, with a single antigenic type that does not cross react with other members of the togavirus group. Rubella virus is relatively unstable and is inactivated by lipid solvents, trypsin, formalin, ultraviolet light, low pH, heat, and amantadine.

This preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE with sucrose. The preparation is free of detergent. Residual non-viral protein comes from host tissue and culture medium

Rubella; Rubella Virus Virion; Group IV; Togaviridae; Rubivirus; Rubella virus

Rubella K1S antigen is inactivated by exposing the material to UV light. This method was chosen to maximize loss of infectivity with a minimum loss of antigenicity.