Набор для анализа активности Общей супероксиддисмутазы / Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

• Detection method: Colorimetric method

 

Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

 

 

Performance characteristics

Synonyms	T-SOD
Sample type	Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity	0.2 U/mL
Detection range	0.2 -14.4 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 3.7%Average intra-assay CV: 2.9%
Other instruments required	Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	Reagent 3: -20℃, others: 2-8℃
Valid period	12 months

Images

The activity of SOD increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced SOD activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.

Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Total superoxide dismutase (T-SOD) activity of fungal spore was determined using T-SOD activity assay kit (E-BC-K020-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	3-5
Rat serum	20-30
Urine	1
Human hydrothorax	2
Cell culture supernatant	2-3
10% Rat liver tissue homogenate	340-370
10% Rat heart tissue homogenate	80-100
10% Rat kidney tissue homogenate	100-120
10% Rat brain tissue homogenate	50-100
10% Plant tissue homogenate	5-10
HepG2 cells (3 mgprot/mL)	30-40

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).