Набор для анализа активности каталазы / Catalase (CAT) Activity Assay Kit

• Detection method: Colorimetric method

Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms	CAT
Sample type	Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity	1.12 U/mL
Detection range	1.12 -150 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 7.700%Average intra-assay CV: 3.900%
Other instruments required	Micropipettor, Vortex Mixer, Incubator
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	6 months

Images

The activity of CAT increased significantly after treated with melatonin in 5 μM of cadmium. (*P<0.05)

D Bartolini et al investigate the mechanism of melatonin protecting porcine pre-pubertal Sertoli cell from cadmium through regulating Nrf2 acivity. Catalase (CAT) activity of porcine pre-pubertal Sertoli cell was determined using CAT activity assay kit (E-BC-K031-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.12 -150 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
293T supernatant	1
10% Rat heart tissue homogenization	50-100
10% Rat liver tissue homogenization	100-200
10% Rat spleen tissue homogenization	50-100
Mouse serum	1
10% Epipremnum aureum tissue homogenization	1-2
10% Rat lung tissue homogenization	50-100
10% Rat kidney tissue homogenization	50-100
10% Rat brain tissue homogenization	20-50

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).