Набор для колориметрического анализа общего железа / Total Iron Colorimetric Assay Kit

Detection instrument: Microplate reader (590-600 nm, optimum wavelength: 593 nm）

Detection method: Colorimetric method

Detection principle

Under the action of reductant, iron ions in samples can be reduced into ferrous ions (Fe2+). The latter then bind to probe and form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.

Performance characteristics

Sample type	serum, tissue
Sensitivity	0.4 μmol/L
Detection range	0.4-50 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	80min
Precision	Average inter-assay CV: 1.500%Average intra-assay CV: 1.300%
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.4-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1-2
Rat serum	1
10% Mouse liver tissue homogenate	2-3
10% Rat lung tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat spleen tissue homogenate	2-3
10% Epipremnum aureum leaf tissue homogenate	1

Note: The diluent is reagent 1.

Информация для заказа

Область использования: Ferrous iron (ионы железа)	Производство:	Elabscience
	Метод:
	Объем:	80 тестов
	Кат. номер:	E-BC-K772-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для колориметрического анализа общего железа / Total Iron Colorimetric Assay Kit. Примечание: