Набор для колориметрического анализа восстановленного глутатиона (GSH) / Reduced Glutathione (GSH) Colorimetric Assay Kit

• Detection method: Colorimetric method

Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm.

 

Performance characteristics

Synonyms	GSH
Sample type	Serum,plasma,tissue,cells
Sensitivity	0.26 mg GSH/L
Detection range	0.26-122.8 mgGSH/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 2.400%Average intra-assay CV: 1.800%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

 

 

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of pre-experiment and the detection range ( 0.26-122.8 mg GSH/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
10% Mouse brain tissue homogenization	1
10% Rat liver tissue homogenization	1
Human plasma	1
Rat plasma	1
10% Carrot tissue homogenization	1
293T supernatant	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).