Набор для колориметрического анализа восстановленного глутатиона (GSH) / Reduced Glutathione (GSH) Colorimetric Assay Kit

Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.

 

 

Performance characteristics

Synonyms	GSH
Sample type	Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity	2 μmol/L
Detection range	2-100 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	25 min
Precision	Average inter-assay CV: 3.200%Average intra-assay CV: 1.900%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

 

 

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
10% Mouse brain tissue homogenization	1
10% Mouse liver tissue homogenization	1
Hela cell  homogenization（0.999 mgprot/mL）	1
Rat serum	1
Rat plasma	1
Mouse serum	1
10% Carrot tissue homogenization	1
293T supernatant	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).