Набор для анализа активности глутатионпероксидазы (GSH-Px) / Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

Performance characteristics

Synonyms	GSH-PX
Sample type	Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity	17.17 U
Detection range	17.17-518.32 U
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 6.200%Average intra-assay CV: 2.400%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

 

 

Dilution of sample

The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	3-5
Rat serum	5-8
10% Mouse brain tissue homogenate	1
10% Rat liver tissue homogenate	30-60
HepG2 cell homogenate (5 mgprot/mL)	1
10% Plant tissue homogenate	1-5

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).