Набор для флуорометрического анализа активных форм кислорода (АФК) / Reactive Oxygen Species (ROS) Fluorometric Assay Kit
- Detection instrument: Fluorescence Microplate reader/Fluorescence Spectrophotometer /Flow Cytometry(Ex/Em=500 nm/525 nm)
- Detection method: Fluorescence method
Detection principle
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Performance characteristics
| Synonyms |
ROS, DCFH-DA, DCFH, DCF |
| Sample type |
Fresh tissue,cultured cells |
| Detection method |
Fluorescence method |
| Assay type |
Cell-based (quantitative) |
| Assay time |
60 min |
| Other instruments required |
Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Storage |
Reagent 1: -20℃, Reagent 2: 2-8℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 96 тестов | | Кат. номер: | E-BC-K138-F | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для флуорометрического анализа активных форм кислорода (АФК) / Reactive Oxygen Species (ROS) Fluorometric Assay Kit.
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