Набор для колориметрического анализа цистеина (Cys) / Cysteine (Cys) Colorimetric Assay Kit
- Detection instrument: Microplate reader(600-620 nm,optimum wavelength: 600 nm)
- Detection method: Colorimetric method
Detection principle
Phosphotungstic acid can be reduced by Cys and form tungsten blue, which has an absorption peak at 600 nm. Cys content can be calculated with the absorbance at 600 nm.
Performance characteristics
| Synonyms |
Cys |
| Sample type |
Serum,plasma,animal tissue,cells |
| Sensitivity |
0.03 mmol/L |
| Detection range |
0.07-2.0 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
30 min |
| Precision |
Average inter-assay CV: 1.300%Average intra-assay CV: 1.100% |
| Other instruments required |
Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer |
| Other reagents required |
Phosphoric acid |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-2.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Mouse serum
|
1
|
|
10% Rat lung tissue homogenate
|
1
|
|
10% Mouse heart tissue homogenate
|
1
|
|
10% Rat brain tissue homogenate
|
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 80 тестов | | Кат. номер: | E-BC-K352-M | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для колориметрического анализа цистеина (Cys) / Cysteine (Cys) Colorimetric Assay Kit.
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