Набор для анализа активности общей супероксиддисмутазы (T-SOD) (гидроксиламиновый метод) / Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

 

 

Performance characteristics

Synonyms	T-SOD
Sample type	Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity	2.4 U/mL
Detection range	2.4-61 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 5.600%Average intra-assay CV: 5.500%
Other instruments required	Micropipettor, Centrifuge, Incubator
Other reagents required	PBS (0.01 M, pH 7.4), Normal saline (0.9% NaCl)
Storage	Reagent 4: -20℃, others: 2-8℃
Valid period	12 months

 

 

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor	The volume of sample
HepG2 supernatant	1	50 μL
HepG2 cell	8-10	25 μL
Mouse serum	3-5	20 μL
10% Mouse liver tissue homogenate	40-60	20 μL
10% Rat kidney tissue homogenate	15-20	20 μL
Human urine	1	25 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).