Набор для анализа активности CuZn/Mn супероксиддисмутазы (CuZn-SOD/Mn-SOD) (гидроксиламиновый метод) / CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

 

 

 

Performance characteristics

Synonyms	CuZn-SOD,Mn-SOD,T-SOD
Sample type	Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity	1.35 U/mL
Detection range	1.35-62 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 9.600%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	Reagent 4: -20℃, others: 2-8℃
Valid period	12 months

 

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	5-10
Rat serum	6-15
Urine	2-3
Human hydrothorax	2-3
10% Mouse liver tissue homogenate	100-200
10% Mouse brain tissue homogenate	20-30
10% Mouse kidney tissue homogenate	50-120
10% Rat kidney tissue homogenate	50-120
HepG2 cells (5.21mgprot/mL)	15-25
Cell culture supernatant	1

Note: The diluent is normal saline (0.9% NaCl).