Набор для колориметрического анализа малонового диальдегида (MDA) (образцы клеток) / Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

 

Performance characteristics

Synonyms	MDA
Sample type	Cells
Sensitivity	0.29 nmol/mL
Detection range	0.29-100 nmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	65 min
Precision	Average inter-assay CV: 3.500%Average intra-assay CV: 3.300%
Other instruments required	Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required	Absolute ethanol
Storage	2-8℃
Valid period	12 months

 

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-100 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
A549 cells	1
HepG2 cells	1
293T cells	1

Note: The diluent is reagent 5.