MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
Synonyms |
MDA |
Sample type |
Cells |
Sensitivity |
0.29 nmol/mL |
Detection range |
0.29-100 nmol/mL |
Detection method |
Colorimetric method |
Assay type |
Quantitative |
Assay time |
65 min |
Precision |
Average inter-assay CV: 3.500%Average intra-assay CV: 3.300% |
Other instruments required |
Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
Other reagents required |
Absolute ethanol |
Storage |
2-8℃ |
Valid period |
12 months |
![](https://www.LD.ru/w/uploads/2024/mald2-D3E95E.png)
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-100 nmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type
|
Dilution factor
|
A549 cells
|
1
|
HepG2 cells
|
1
|
293T cells
|
1
|
Note: The diluent is reagent 5.