- Detection method: Fluorescence method
Detection principle
Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.
Performance characteristics
| Synonyms |
CAT |
| Sample type |
Serum, plasma, animal tissue |
| Sensitivity |
0.01 U/L |
| Detection range |
0.01-6.51 U/L |
| Detection method |
Fluorescence method |
| Assay type |
Enzyme Activity |
| Assay time |
30 min |
| Precision |
Average inter-assay CV: 6.800%Average intra-assay CV: 3.400% |
| Other instruments required |
Vortex mixer, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-6.51 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Mouse serum
|
60-80
|
|
Mouse plasma
|
60-80
|
|
Rat serum
|
70-80
|
|
Human saliva
|
30-50
|
|
Rat urine
|
30-50
|
|
10% Mouse liver tissue homogenate
|
2500-3000
|
|
10% Mouse lung tissue homogenate
|
200-400
|
|
10% Rat muscle tissue homogenate
|
100-200
|
|
10% Rat brain tissue homogenate
|
40-50
|
|
10% Mouse kidney tissue homogenate
|
2000-2500
|
Note: The diluent is reagent 1.
