Набор для колориметрического анализа перекиси водорода (H2O2) / Hydrogen Peroxide (H2O2) Colorimetric Assay Kit

• Detection method: Colorimetric method

Detection principle

Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm.

Performance characteristics

Synonyms	H2O2
Sample type	Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity	1.5 mmol/L
Detection range	1.5-150 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 2.700%Average intra-assay CV: 1.300%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.5-150 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse liver tissue homogenization	5-10
Human serum	1
10% Green pepper tissue homogenization	1
Rat serum	1
Mouse serum	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).