- Detection method: Fluorescence method
Detection principle
In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.
Performance characteristics
| Sample type |
Serum, plasma, tissue, cells |
| Sensitivity |
0.02 μmol/L |
| Detection range |
0.02-10 μmol/L |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
50 min |
| Precision |
Average inter-assay CV: 3.600%Average intra-assay CV: 1.100% |
| Other instruments required |
Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Storage |
Reagent 5 & Reagent 6, 2-8℃; others, -20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.02-10 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1-3
|
|
Rat serum
|
1-4
|
|
Mouse serum
|
1-4
|
|
Mouse plasma
|
1-2
|
|
Porcine serum
|
1-3
|
|
HepG2 cell culture supernatant
|
1
|
|
10% Mouse liver tissue homogenate
|
1-3
|
|
10% Mouse brain tissue homogenate
|
1-3
|
|
10% Rat lung tissue homogenate
|
1-3
|
|
HepG2 cell homogenate (6.44 gprot/L)
|
1-2
|
Note: The diluent is reagent 1.
