NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.
Performance characteristics
| Synonyms |
NO |
| Sample type |
Serum,plasma,tissue |
| Sensitivity |
0.97 μmol/L |
| Detection range |
0.97-700 μmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
80 min |
| Precision |
Average inter-assay CV: 5.200%Average intra-assay CV: 3.400% |
| Other instruments required |
Vortex mixer, Centrifuge, Analytical Balance, Micropipettor |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
Reagent 6: -20℃, others: 2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-700 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Human plasma
|
1
|
|
10% Mouse liver tissue homogenization
|
1
|
|
Rat serum
|
1
|
|
Rat plasma
|
1
|
|
10% Epipremnum aureum tissue homogenization
|
1
|
Note: The diluent is double distilled water, normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
