Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases.
Performance characteristics
| Synonyms |
MPO |
| Sample type |
Serum, plasma, animal tissue |
| Sensitivity |
0.001 U/L |
| Detection range |
0.001 - 1.26 U/L |
| Detection method |
Fluorescence method |
| Assay type |
Enzyme Activity |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 5.400%Average intra-assay CV: 1% |
| Other instruments required |
Micropipette, Vortex mixer, Water bath |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.001 - 1.26 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Porcine serum
|
5-10
|
|
Rabbit serum
|
3-5
|
|
Rat serum
|
2-5
|
|
Mouse serum
|
10-20
|
|
Mouse plasma
|
30-50
|
|
Horse serum
|
2-5
|
|
10% Rat heart tissue homogenate
|
1
|
|
10% Rat lung tissue homogenate
|
1
|
Note: The diluent is reagent 1.
