- Detection method: Colorimetric method
Manual
The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
![](https://www.LD.ru/w/uploads/2024/k117-1E5B7E4.png)
Performance characteristics
Sample type |
Serum,plasma,hydrothorax,cell culture supernatant,tissue |
Sensitivity |
0.02 nmol/mgprot |
Detection range |
0.02-10 nmol/mgprot |
Detection method |
Colorimetric method |
Assay type |
Quantitative |
Assay time |
240 min |
Precision |
Average inter-assay CV: 8.600%Average intra-assay CV: 4.500% |
Other instruments required |
Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
Other reagents required |
Absolute ethanol, Ethyl acetate |
Storage |
2-8℃ |
Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment. The sample can be detected by this kit when the protein content of samples is ranged from 1-10 mg/mL.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type
|
Dilution factor
|
Human serum
|
8-10
|
Mouse serum
|
8-10
|
10% Rat liver tissue homogenization
|
2-3
|
10% Mouse heart tissue homogenization
|
1
|
10% Mouse brain tissue homogenization
|
1
|
Note: The diluent is double distilled water or reagent 1.