Набор для колориметрического анализа карбонила белка (образцы тканей и сыворотки) / Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)

• Detection method: Colorimetric method

Manual

 

The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.

 

 

Performance characteristics

Sample type	Serum,plasma,hydrothorax,cell culture supernatant,tissue
Sensitivity	0.02 nmol/mgprot
Detection range	0.02-10 nmol/mgprot
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	240 min
Precision	Average inter-assay CV: 8.600%Average intra-assay CV: 4.500%
Other instruments required	Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Other reagents required	Absolute ethanol, Ethyl acetate
Storage	2-8℃
Valid period	12 months

Dilution of sample

 

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment. The sample can be detected by this kit when the protein content of samples is ranged from 1-10 mg/mL.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	8-10
Mouse serum	8-10
10% Rat liver tissue homogenization	2-3
10% Mouse heart tissue homogenization	1
10% Mouse brain tissue homogenization	1

Note: The diluent is double distilled water or reagent 1.