Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)

Detection instrument: Microplate reader(405-425 nm)

Detection method: Colorimetric method

Detection principle

The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,urine,saliva,tissue,cells
Sensitivity	0.047 mmol/L
Detection range	0.047-1.50 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 4.100%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), 60% Ethanol
Storage	Reagent5:-20℃,Others:2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.047-1.50 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat brain tissue homogenization	1
10% Rat liver tissue homogenization	1
10% Rat kidney tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1
Human serum	1
Human saliva	1
Human urine	1
Rat serum	1

Note: When the sample was water soluble, the diluent is PBS (0.01 M, pH 7.4);

When the sample is insoluble, the diluent is 80% ethanol.

Информация для заказа

Область использования: Набор для анализа общей антиоксидантной способности / Total Antioxidant Capacity (T-AOC) Assay Kit	Производство:	Elabscience
	Метод:
	Объем:	80 тестов
	Кат. номер:	E-BC-K219-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method). Примечание: