- Detection method: Colorimetric method
Manual
Detection principle
The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.
Performance characteristics
| Synonyms |
T-AOC |
| Sample type |
Serum,plasma,urine,saliva,tissue,cells |
| Sensitivity |
0.05 mmol/L |
| Detection range |
0.05-1.00 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
20 min |
| Precision |
Average inter-assay CV: 5%Average intra-assay CV: 4.100% |
| Other instruments required |
Micropipettor, Centrifuge, Vortex mixer |
| Other reagents required |
Double distilled water, 1 × PBS, 80% Ethanol |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-1.00 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
20% Tomato tissue homogenization
|
2-5
|
|
10% Mouse heart tissue homogenization
|
8-12
|
|
10% Mouse liver tissue homogenization
|
8-12
|
|
10% Mouse lung tissue homogenization
|
8-12
|
|
Human saliva
|
2-5
|
|
Human urine
|
15-30
|
|
Human serum
|
15-30
|
|
Human plasma
|
8-15
|
Note: The diluent is 1 × PBS or 80% ethanol.
