Набор для колориметрического анализа глутамина (Gln) / Glutamine (Gln) Colorimetric Assay Kit

Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

Detection principle

Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms	Gln
Sample type	serum (plasma), tissue, cells and cell culture supernatan
Sensitivity	0.036 mmol/L
Detection range	0.036–2.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	70 min
Precision	Average inter-assay CV: 4.700%Average intra-assay CV: 3.200%
Other instruments required	Incubator, 50 KD Ultrafiltration tube
Other reagents required	Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Информация для заказа

Область использования: Аминокислоты / Amino Acids Купроптоз / Cuproptosis	Производство:	Elabscience
	Метод:
	Объем:	80 тестов
	Кат. номер:	E-BC-K853-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для колориметрического анализа глутамина (Gln) / Glutamine (Gln) Colorimetric Assay Kit. Примечание: