Набор для анализа активности альфа-кетоглутаратдегидрогеназы (alfa-KGDH) / alfa-Ketoglutarate Dehydrogenase(alfa-KGDH) Activity Assay Kit

Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

Detection principle

α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm

Performance characteristics

Synonyms	α-KGDH
Sample type	serum, plasma, animal and plant tissue
Sensitivity	8.3 U/L
Detection range	8.3-42.3 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 8.500%Average intra-assay CV: 1.500%
Other instruments required	Incubator, Centrifuge
Storage	-20℃
Valid period	12 months

Информация для заказа

Область использования: Купроптоз / Cuproptosis Цикл трикарбоновой кислоты / Tricarboxylic Acid（TCA）Cycle	Производство:	Elabscience
	Метод:
	Объем:	40 тестов
	Кат. номер:	E-BC-K083-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для анализа активности альфа-кетоглутаратдегидрогеназы (alfa-KGDH) / alfa-Ketoglutarate Dehydrogenase(alfa-KGDH) Activity Assay Kit. Примечание: