- Detection method: Colorimetric method
Manual
Detection principle
In acidic condition, Cr (Ⅵ) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm.
Performance characteristics
| Synonyms |
CA |
| Sample type |
Tissue,mitochondria,other liquid samples |
| Sensitivity |
0.05 mmol/L |
| Detection range |
0.05-5.0 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 5.400%Average intra-assay CV: 4.200% |
| Other instruments required |
Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-5.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
4-6
|
|
10% Mouse kidney tissue homogenate
|
2-4
|
|
10% Rat kidney tissue homogenate
|
1
|
|
10% Mouse heart tissue homogenate
|
2-4
|
|
10% Mouse brain tissue homogenate
|
1
|
|
10% Mouse liver tissue homogenate
|
1
|
Note: The diluent is reagent 1.
