Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The activity of mitochondrial complex I can be quantified by measure the change OD value at 340 nm.
Performance characteristics
Synonyms
NADH-CoQ Reductase
Sample type
cell
Sensitivity
2.85 U/L
Detection range
2.85-119.04 U/L
Detection method
Colorimetric method
Assay type
Enzyme Activity
Assay time
30 min
Precision
Average inter-assay CV: 5.0-8.0%Average intra-assay CV: 3.0-4.0%