Набор для анализа активности митохондриального комплекса I (NADH-CoQ-редуктаза) / Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit

Detection instrument: Microplate reader (340 nm)

Detection method: Colorimetric method

Detection principle

Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.

Performance characteristics

Sample type	animal tissue
Sensitivity	15.58 U/L
Detection range	15.58-224.2 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	15 min
Precision	Average inter-assay CV: 8.400%Average intra-assay CV: 0.800%
Other instruments required	Centrifuge, 37℃ incubator
Other reagents required	Anhydrous ethanol (AR)
Storage	-20℃
Valid period	12 months

Информация для заказа

Область использования: митохондриальные комплексы / Mitochondrial Complex	Производство:	Elabscience
	Метод:
	Объем:	48 тестов
	Кат. номер:	E-BC-K149-M
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор для анализа активности митохондриального комплекса I (NADH-CoQ-редуктаза) / Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit. Примечание: