- Detection method: Colorimetric method
Manual
Detection principle
Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.
Performance characteristics
| Sample type |
animal tissu |
| Sensitivity |
20 U/mL |
| Detection range |
20-2000 U/mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
70 min |
| Precision |
Average inter-assay CV: 6.500%Average intra-assay CV: 5.400% |
| Other instruments required |
Micropipette, Vortex mixer, Centrifuge |
| Other reagents required |
PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (20-2000 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
20% Rat ileum tissue homogenate
|
1
|
|
20% Rat stomach tissue homogenate
|
1
|
|
20% Rat liver tissue homogenate
|
1
|
Note: The diluent is PBS (0.01 M, pH 7.4).
