Набор для анализа активности аскорбатпероксидазы (APX) / Ascorbate Peroxidase (APX) Activity Assay Kit
- Detection instrument: Spectrophotometer(290 nm)
- Detection method: Colorimetric method
Manual
Detection principle
Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.
Performance characteristics
| Synonyms |
APX |
| Sample type |
Plant tissue |
| Sensitivity |
0.071 U/g tissue |
| Detection range |
0.071-47 U/g tissue |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 6.400%Average intra-assay CV: 4.800% |
| Other instruments required |
Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 96 тестов | | Кат. номер: | E-BC-K353-S | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для анализа активности аскорбатпероксидазы (APX) / Ascorbate Peroxidase (APX) Activity Assay Kit.
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