- Detection method: Colorimetric method
Manual
Detection principle
In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorptivity of the sample extract at 510 nm.
Performance characteristics
| Sample type |
Plant tissue |
| Sensitivity |
0.315 μg/mL |
| Detection range |
0.315-150 μg/mL |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
60 min |
| Precision |
Average inter-assay CV: 2.200%Average intra-assay CV: 1.900% |
| Other instruments required |
Vortex mixer, Micropipettor, Air oven , Crusher, Ultrasonic cell processor |
| Other reagents required |
60% Ethanol |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.315-150 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Camphor leaves tissue homogenization
|
8-15
|
|
Carrot tissue homogenization
|
2-5
|
|
Green pepper tissue homogenization
|
1
|
Note: The diluent is 60% Ethanol.
