Набор для колориметрического анализа железа / Iron Colorimetric Assay Kit
- Detection instrument: Microplate reader(510-530 nm,optimum wavelength: 520 nm)
- Detection method: Colorimetric method
Manual
Detection principle
Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Performance characteristics
| Synonyms |
Fe |
| Sample type |
Serum,tissue |
| Sensitivity |
0.08 mg/L |
| Detection range |
0.29-10 mg/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
25 min |
| Precision |
Average inter-assay CV: 2.800%Average intra-assay CV: 1.200% |
| Other instruments required |
Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer |
| Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Информация для заказа| Область использования: | Производство: | Elabscience | | Метод: | | | Объем: | 80 тестов | | Кат. номер: | E-BC-K139-M | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: Набор для колориметрического анализа железа / Iron Colorimetric Assay Kit.
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