- Detection method: Colorimetric method
Manual
Detection principle
The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of β-amylase, and then the enzyme activity of α-amylase is determined.
Performance characteristics
| Sample type |
animal and plant tissue |
| Sensitivity |
0.0097 U/mL |
| Detection range |
0.0097-0.3474 U/mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
40 min |
| Precision |
Average inter-assay CV: 3.900%Average intra-assay CV: 3% |
| Other instruments required |
Micropipettor, Multi-channel pipettor, Incubator, Vortex mixer, Centrifuge |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-34.74 U/g tissue).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
1% Epipremnum aureum tissue homogenate
|
1
|
|
1% Green pepper tissue homogenate
|
1
|
|
1% Corn grain tissue homogenate
|
1
|
|
1% Daucus carota tissue homogenate
|
1
|
Note: The diluent is double distilled water.
