Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly bymeasuring the OD value at 410 nm.
Performance characteristics
Synonyms
PPO
Sample type
Plant tissue
Detection method
Colorimetric method
Assay type
Enzyme Activity
Assay time
25 min
Precision
Average inter-assay CV: 8.700%Average intra-assay CV: 4.600%
Other instruments required
Tubes, Micropipette, Vortex mixer, 100℃ Water bath