- Detection method: Fluorescence method
Manual
Detection principle
Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Performance characteristics
| Sample type |
Plant tissue |
| Sensitivity |
0.15 μmol/L |
| Detection range |
0.15-15 μmol/L |
| Detection method |
Fluorescence method |
| Assay type |
Quantitative |
| Assay time |
70 min |
| Precision |
Average inter-assay CV: 6.500%Average intra-assay CV: 2.300% |
| Other instruments required |
Micropipettor, Incubator, Water bath, Centrifuge |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.15-15 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Corn tissue homogenate
|
1500
|
|
10% Potato tissue homogenate
|
300-500
|
|
10% Tomato tissue homogenate
|
200-300
|
|
10% Macrophanerophytes leaf tissue homogenate
|
200-400
|
|
10% Carrot tissue homogenate
|
1500
|
|
10% Onion tissue homogenate
|
500-1000
|
|
10% Green pepper tissue homogenate
|
500-1000
|
|
10% Bush leaves tissue homogenate
|
20-50
|
Note: The diluent is reagent 1 working solution.
