- Detection method: Colorimetric method
Manual
Detection principle
Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324 nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase.
Performance characteristics
| Sample type |
plant tissue |
| Sensitivity |
0.3 U/mL |
| Detection range |
0.3-350 U/mL |
| Detection method |
Colorimetric method |
| Assay type |
Enzyme Activity |
| Assay time |
30 min |
| Precision |
Average inter-assay CV: 8.200%Average intra-assay CV: 1.100% |
| Other instruments required |
Test tube, Micropipettor, Vortex mixer, Optical path cuvette (1 mL of volume, 1 cm optical diameter) |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.3-350 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
10% Oxalis corniculata tissue homogenate
|
1
|
|
10% Ginkgo biloba tissue homogenate
|
1
|
|
10% Cactus tissue homogenate
|
1
|
|
10% Bamboo leaf tissue homogenate
|
1
|
|
10% Osmanthus fragrans leaf tissue homogenate
|
4-8
|
|
10% Camphor trees leaf tissue homogenate
|
3-5
|
Note: The diluent is reagent 1.
