- Detection method: Colorimetric method
Manual
Detection principle
Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Under the action of POD, hydrogen peroxide reacts with chromogenic substances to produce colored products. There is a specific absorption peak at 550 nm, and the color depth is proportional to the content of oxalate.
Performance characteristics
| Sample type |
animal urine, serum, plasma， plant tissue |
| Sensitivity |
0.02 mmol/L |
| Detection range |
0.02-1 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
35 min |
| Precision |
Average inter-assay CV: 4.700%Average intra-assay CV: 3% |
| Other instruments required |
Micropipettor, Centrifuge, 37℃ incubator |
| Storage |
-20℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.02-1 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human urine
|
1-2
|
|
Human plasma
|
1
|
|
10% Epipremnum aureum tissue homogenate
|
2-3
|
|
Rats plasma
|
1
|
Note: The diluent is double distilled water.
