- Detection method: Colorimetric method
Manual
Detection principle
The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.
Performance characteristics
| Synonyms |
TG |
| Sample type |
Serum,plasma,tissue |
| Sensitivity |
0.19 mmol/L |
| Detection range |
0.19-8.0 mmol/L |
| Detection method |
Colorimetric method |
| Assay type |
Quantitative |
| Assay time |
50 min |
| Precision |
Average inter-assay CV: 6.700%Average intra-assay CV: 2.400% |
| Storage |
2-8℃ |
| Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.19-8.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type
|
Dilution factor
|
|
Human serum
|
1
|
|
Mouse serum
|
1
|
|
Rat plasma
|
1
|
|
10% Rat liver tissue homogenate
|
1
|
|
10% Rat kidney tissue homogenate
|
1
|
|
10% Mouse brain tissue homogenate
|
1
|
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples.
