Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
![](https://www.LD.ru/w/uploads/2024/k004-3C01AC5.png)
Performance characteristics
Synonyms |
FC |
Sample type |
Serum, plasma, tissue |
Sensitivity |
0.07 mmol/L |
Detection range |
0.07-24 mmol/L |
Detection method |
Colorimetric method |
Assay type |
Quantitative |
Assay time |
20 min |
Precision |
Average inter-assay CV: 5.200%Average intra-assay CV: 1.900% |
Other instruments required |
Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required |
Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR) |
Storage |
2-8℃ |
Valid period |
12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-24 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type
|
Dilution factor
|
Human plasma
|
1
|
Human serum
|
1
|
Rat serum
|
1
|
Mouse serum
|
1
|
Rabbit serum
|
1
|
10% Rat kidney tissue homogenate
|
1
|
Note: The diluent is normal saline (0.85% NaCl) or PBS (0.01 M, pH 7.4).